How to construct signatures from iLINCS datasets

The transcriptomics or proteomics signature is constructed by comparing expression levels of two groups of samples (treatment group and baseline group) using Empirical Bayes linear model implemented in the limma package. For the GREIN collection of GEO RNA‐seq datasets, the signatures are constructed using the negative‐binomial generalized linear model as implemented in the edgeR package.

Last updated on 26th May 2020