Global chromatin profiling technology is a mass spectrometry-based assay to identify and quantify post-translational modifications on histone proteins from bulk chromatin. The assay quantifies around 60 different combinations of histone modifications on H3, with even more possible combinations on H4, H2A (including isoforms), and H2B. GCP can be considered as orthogonal to ChIP-Seq. Instead of characterizing one histone mark at a time with locus resolution across the genome, one can profile almost all combinations of histone marks together in bulk chromatin with no genomic resolution. The technique is particularly useful in assessing the functional consequences of genetic mutations to the epigenetic machinery and can be employed to make connections among biological conditions and perturbations.

Keywords: P100 | Proteomics | LINCS | asdf |

Category: assays

Last updated on 30th Oct 2018